| 1. | He had his own phrenic nerve tied to revive the memory of what happened to the patient and to test the effectiveness of phrenicectomy .
他又扎起自己的膈神经，使自己重新体会病人的感觉，同时检验膈胃神经切断术的效力。 |
| 2. | Anatomic structures related to the blocking of phrenic nerve
膈神经阻滞入路相关解剖结构 |
| 3. | An applied anatomical study of choosing transfer course of phrenic nerve harvested from thoracic cavity
选择进胸取膈神经移位路径的应用解剖学研究 |
| 4. | The toxin can reversible block the neuromuscular transmission in an isolated mouse phrenic nerve - diaphragm preparation and also is a neurotoxin ( n - type ca2 + channels inhibitor ) which binds to the postsynaptic nicotinic acetylcholine receptor ( nachr )
溶液构象研究表明，它采取自然界中其它蛋白质多肽普遍采用的一种紧凑、致密的抑制剂胱氨酸结模体( ick ) 。 |
| 5. | We also synthesized w - ctx mviia and studied its renaturation under air oxidizing and gsh - gssg condition . we researched the physiological activities of the renatured peptides on isolated mouse phrenic nerve - diaphragm preparations or on isolated mouse spermaduct preparations
另外还合成了- ctxmv a ，并用空气氧化法和谷胱甘肽法，研究了多种条件下的复性情况。 |
| 6. | After renaturation , the biological activity of mviia is nearly 100 % of that of natural one ; the activity of hm - 1227 is less than t | iat of hwtx - i ; however , hm - xw cannot block the neuromuscul ar transmission in an isolated mouse phrenic nerve - diaphragm preparation
复性后的mv a摘要生物学活性接近天然mvlla的100 % ;嵌合体hm一1227的活性小于hwtx一i的15 % ;而嵌合体fd以一xw不能阻断小鼠隔神经一隔肌的接头传递。 |
| 7. | The sequence of hntx - v had been determined by 491 sequencer : nh2 - eclgfgkgcnpsdqccksanlvcsrkhrwckyei - cooh , in which there were 35 amino acid residues and six cys . the toxin could block neuromuscular transmission in an isolated mouse phrenic nerve diaphragm preparation . hntx - v is a natural mutant of hntx - iv , for there is only one different residue ( ala20ser )
Hntx -经491测序仪测得其氨基酸组成为： nh2 - eclgfgkgcnpsdqccksanlvcsrkhrwckyei - cooh ，含35个氨基酸残基， 6个半胱氨酸全部参与了二硫键的形成，该毒素对小鼠膈神经-膈肌标本有阻遏作用。 |
| 8. | 46da , respectively . the sequences of the two toxins are determined and found to consist of 34 and 36 amino acid residues , respectively . both of the two sequences include six cys residues , which form three disulphide bonds . jztx - can block neuromuscular transmission in an isolated mouse phrenic nerve diaphragm
Jztx -对电刺激诱导的小鼠膈神经-膈肌收缩产生抑制作用，浓度为1 . 0 10 ~ ( - 6 ) mol / l时的阻断时间为15分钟左右，对钠通道和延迟调整型钾通道的激活和失活没有影响。 |
| 9. | Mass spectrometry of synthetic hw - ma and rgd - hw are in full agreement with those speculated theoretically , which proves the success of peptide synthesis and refold . on isolated mouse phrenic nerve - diaphragm preparations , hw - ma can block the neuromuscular transmission in 35 minutes or so ( l 10 - 5 g / ml ) , its biological activity shows 73 % decrease comparing with biological activity of native hwtx - i . it proves t hat the protein engineering of synthetic chimera hwtx - i has gained success to some extent , although it did not achieve our expectations . thus it proved that hwtx - i can be using as natural scaffold for protein engineering . and also emphasized the importance of " local stereo circumstances " of activity site when the foreign activity site was transferred into a natural scaffold
浓度为1 / 1059 / ml的hw一ma突变体能可逆阻断小白鼠隔神经书高肌的接头传递，阻断时一间为35min左右，与天然hwtx一i比较，生物学活性下降3一4倍，说明合成的突变体改造获得了一定的成功，尽管与我们预期的目标有一定的差距，从而证明hwtx一i可以作为蛋白质工程研究的天然分子骨架，同时也强调了往天然分子骨架中转移外源活性位点时维持活性位点“局部立体环境”的重要性。 |
